High-throughput screening (HTS) is seen as one of the most promising technologies to facilitate
biomedical studies and pharmaceutical discoveries. Although large varieties of in vitro HTS
technologies have opened great opportunities to this society, the speed of improvement has
been limited by lack of advanced tools for in vivo screening on whole complex organisms, such
as vertebrates. To address this issue, a high-throughput platform as a vertebrate total
analysis/screening system (V-TAS) is proposed. This platform consists of two independent parts:
an automated imaging system and an automated microinjection system. These two systems are
designed for most of general high-content high-throughput pharmaceutical and genetic screens
on whole zebrafish larvae, and therefore, are well-modularized for adapting different situations.
Furthermore, to demonstrate the capability of V-TAS, a screen of lipidoid library on thousands
of animals was conducted. Very limited damage to the larvae was shown during the screening.
In the end, the author also validated the hits discovered by V-TAS can be applied to more
advanced animal models such as rats, and be more predictable than cell-based assays.
Thesis Supervisor: Mehmet Fatih Yanik
Title: Associate Professor of Electrical Engineering and Computer Science